Fig. 2. CaBP-9k knockout causes Parkinson's disease in mice. (A) Immunofluorescence staining for α-synuclein and TH in dopaminergic cells in the SNc and VTA of old CaBP-9k KO mice. Scale bar= 500 µm. (B) Quantification of A. n = 7 mice for wild-type; n = 6 mice for CaBP-9k KO mice. (C) SNc and VTA sections from old CaBP-9k KO mice were immunostained with TH antibodies. Scale bar= 40 µm. (D) Quantification of C. n = 10 for mice for each group. (E) mRNA levels of dopamine-related genes were assessed in brain lysates from old wild-type and CaBP-9k KO mice by real-time PCR. n = 5 for mice for each group. mRNA levels were normalized to the GAPDH level. Data shown are the means ± SEMs and were analysed by two-tailed unpaired Student's t-tests.